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Aptamer‐based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cells
Author(s) -
Karpik Agnieszka E.,
Crulhas Bruno P.,
Rodrigues Carolina B.,
Castro Gustavo R.,
Pedrosa Valber A.
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700318
Subject(s) - aptamer , lncap , biosensor , prostate cancer , muc1 , cancer biomarkers , linear range , nanotechnology , conjugated system , materials science , chemistry , detection limit , combinatorial chemistry , cancer , chromatography , microbiology and biotechnology , mucin , biochemistry , biology , medicine , composite material , polymer
Electrochemical aptasensors can detect cancer biomarkers such as mucin 1 (MUC1) to provide point‐of‐care diagnosis that is low‐cost, specific and sensitive. Herein, a DNA hairpin containing MUC1 aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self‐assembly. The fabrication process was characterized by scanning electron microscopy, X‐ray spectroscopy analysis and electrochemistry techniques. The results evidenced a stable and sensitivity sensor presenting wide linear detection range (0.65–110 ng/mL). Therefore, it was able to precisely detect MUC1 production patterns in normal (RWPE‐1) and prostate cancer cells (LNCaP and PC3). The biosensor has ability to detect MUC1 in complex samples being an efficient and useful platform for cancer diagnosing in early stages and for physiological applications such as cancer treatment monitoring.