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Real‐time Selective Detection of Hydrogen Peroxide Based on a Tantalum Deposited Pencil Lead Electrode for Evaluation of Enzyme Activities
Author(s) -
Jo Ara,
Lee Youngmi,
Lee Chongmok
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700315
Subject(s) - amperometry , ascorbic acid , hydrogen peroxide , glucose oxidase , chemistry , tantalum , electrode , nuclear chemistry , inorganic chemistry , catalase , biosensor , electrochemistry , biochemistry , organic chemistry , antioxidant , food science
In this study, we have carried out electrodeposition of tantalum (Ta) nanostructures on pencil lead electrode in non‐aqueous media at room temperature by applying a constant potential. The deposited Ta on pencil lead was examined for the catalytic effect regarding hydrogen peroxide (H 2 O 2 ) reduction with voltammetry and amperometry. Ta/pencil lead electrode exhibited amperometric sensitivity of 0.317 μA mM −1 cm −2 and fast response time of 0.75 s, where selective detection of H 2 O 2 was fulfilled without interruption from common electroactive biomaterials such as O 2 , uric acid, ascorbic acid, dopamine, acetamidophenol, and glucose. For practical applications, the dynamic concentration changes of H 2 O 2 during catalase and glucose oxidase‐involved reactions, either eliminating or producing H 2 O 2 , were successfully traced in real time with as‐prepared electrode. From the kinetics study for catalase and glucose oxidase, we evaluated Michaelis constants ( K m app ) as 7.8 mM for catalase and 37 mM for glucose oxidase, respectively.