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Electrochemical Determination of Label Free BRCA Hybridization by Single Use Antioxidant Modified Electrode
Author(s) -
Muti Merve,
Muti Mihrican
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700256
Subject(s) - caffeic acid , selectivity , electrode , guanine , chemistry , dna , electrochemistry , hybridization probe , combinatorial chemistry , biochemistry , antioxidant , gene , nucleotide , catalysis
Abstract A novel DNA probe based on caffeic acid modified disposable pencil graphite electrodes were developed for the first time for the electrochemical determination of breast cancer gene sequence (BRCA) hybridization. Amino‐linked BRCA probe highly immobilized onto the caffeic acid modified electrode by means of the interaction between the amino group of BRCA probe and the carboxyl group of caffeic acid compared to the bare electrode. 44 % signal enhancement in guanine oxidation signal was measured by caffeic acid modified electrode. Besides, these probes exhibited high selectivity towards its complementary DNA sequences (target). Hybridization between probe and target (BRCA1) was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The selectivity was also tested in the presence of mixture containing the target and one base mismatch BRCA sequences in the same ratio (1 : 1). It can be said this probe can select its complementary from the mixture.