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Oral Cancer Screening Based on Methylation Frequency Detection in hTERT Gene Using Electrochemical Hybridization Assay via a Multi‐electrode Chip Coupled with Ferrocenylnaphthalene Diimide
Author(s) -
Haraguchi Kazuya,
Sato Shinobu,
Habu Manabu,
Yada Naomi,
Hayakawa Mana,
Takahashi Osamu,
Yoshioka Izumi,
Matsuo Kou,
Tominaga Kazuhiro,
Takenaka Shigeori
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201700028
Subject(s) - methylation , telomerase reverse transcriptase , cancer , cancer research , telomerase , electrode , carcinogenesis , lesion , leukoplakia , microbiology and biotechnology , chemistry , gene , biology , medicine , pathology , biochemistry , genetics
Ferrocenylnaphthalene diimide‐based electrochemical hybridization assay via a multi‐electrode chip was applied to detect the methylation frequency in the promoter region of human telomerase reverse transcriptase ( hTERT ) gene for clinical samples from tissues, local exfoliated oral cells from a lesion, or from entire oral cavity after their methylation specific PCRs. These methylation frequencies were increased with cancer progress as the following order: healthy volunteers, oral leukoplakia as precancerous lesion, and oral squamous cell carcinoma (OSCC). Operating characteristic analysis of the obtained current data doesn't only give excellent discrimination ability of OSCC, but also of oral leukoplakia from healthy volunteers for all samples. Sensitivity and specificity was 95 % and 90 %, respectively, which is a comparable with methods in practical use.