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Development and Optimization of an Ion‐selective Electrode for Serotonin Detection
Author(s) -
Bliem Christina,
Fruhmann Philipp,
Stoica AncaIulia,
Kleber Christoph
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600782
Subject(s) - potentiometric titration , electrode , detection limit , analyte , membrane , materials science , plasticizer , analytical chemistry (journal) , graphite , potentiometric sensor , ion , ion selective electrode , chemistry , chromatography , selectivity , composite material , organic chemistry , biochemistry , catalysis
Our studies are focused on the development of novel potentiometric sensors for the quantification of the neurotransmitter serotonin. Therefore, ion‐selective electrodes based on plasticized PVC membranes are applied. The electroactive part of the membrane consists of an ion pair complex formed between the protonated analyte and a carborane anion [Co(1,2‐C 2 B 9 H 11 ) 2 ] − . The analytical performance of the electrode was studied regarding sensitivity, concentration range, limit of detection and potential stability. The ion‐selective electrodes were optimized with respect to the material of the transducing element, as well as the membrane thickness and its composition. Stable, all solid state ISEs could be developed, using the non‐polar plasticizer NPOE and a graphite rod with high surface area as transducing element. We thus achieved a near Nernstian response over three decades of concentration (2.25⋅10 ‐5 ‐1.00⋅10 ‐2 M) and a limit of detection in the μ‐molar range for the optimized electrodes. The electrodes could successfully be miniaturized using carbon based screen printed electrodes.