Premium
A Novel Superoxide Anion Biosensor for Monitoring Reactive Species of Oxygen Released by Cancer Cells
Author(s) -
Crulhas Bruno P.,
Recco Lucas C.,
Delella Flávia K.,
Pedrosa Valber A.
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600767
Subject(s) - biosensor , detection limit , reactive oxygen species , superoxide , dielectric spectroscopy , chemistry , cyclic voltammetry , ethylene glycol , analytical chemistry (journal) , electrochemistry , nuclear chemistry , combinatorial chemistry , nanotechnology , materials science , electrode , chromatography , biochemistry , organic chemistry , enzyme
In this study, novel sensitive and selective hydrogel microstructures to detect superoxide anions released by cancer cells, based on electrochemical biosensors, are proposed. Ferrocene was coupled with superoxide dismutase within a poly(ethylene glycol) diacrylate hydrogel matrix. The pre‐polymer solution was patterned by photolithography in gold microelectrodes fabricated on top of glass slides. The biosensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry, and was able to detect superoxide anions in a wide linear range from 5 to 100 μM, with a low detection limit of 0.001 μM and sensitivity of 14.1 nA μM/mm 2 . Moreover, the biosensor was able to directly detect reactive oxygen species released from prostate cells. Furthermore, the reproducibility, stability and selectivity of the biosensor achieved better results when compared with the previous report, so this methodology can be used in physiological and pathological detection of reactive oxygen species, providing a powerful platform for clinical diagnostics in the future.