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A Biofuel Cell Based on Biocatalytic Reactions of Glucose on Both Anode and Cathode Electrodes
Author(s) -
Koushanpour Ashkan,
Gamella Maria,
Guz Nataliia,
Katz Evgeny
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600706
Subject(s) - glucose oxidase , electrocatalyst , chemistry , hemin , redox , catalysis , electrochemistry , graphene , anode , electrode , materials science , inorganic chemistry , biochemistry , nanotechnology , biosensor , enzyme , heme
Biofuel cells based on electrocatalytic oxidation of NADH and reduction of H 2 O 2 have been prepared using carbon fiber electrodes functionalized with graphene nano‐flakes. The electrochemical oxidation of NADH was catalyzed by Meldola's blue (MB), while the reduction of H 2 O 2 was catalyzed by hemin, both catalysts were adsorbed on the graphene flakes due to their π‐π staking. In the next set of experiments, the MB‐ and hemin‐electrodes were additionally modified with glucose dehydrogenase (GDH) and glucose oxidase (GOx), respectively. The enzyme catalyzed reactions in the presence of glucose, NAD + and O 2 resulted in the production of NADH and H 2 O 2 in situ. The produced NADH and H 2 O 2 were oxidized and reduced, respectively, at the bioelectrocatalytic electrodes, thus producing voltage and current generated by the biofuel cell. The enzyme‐based biofuel cells operated in a human serum solution modelling an implantable device powered from the natural biofluid. Finally, two enzyme‐based biofuel cell connected in series and operating in the serum solution produced electrical power sufficient for activation of an electronic watch used as an example device.