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Gold Nanoparticles Stabilized in β‐Cyclodextrin and Decorated with Laccase Applied in the Construction of a Biosensor for Rutin
Author(s) -
Brugnerotto Patricia,
Silva Tânia Regina,
Brondani Daniela,
Zapp Eduardo,
Vieira Iolanda Cruz
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600697
Subject(s) - biosensor , rutin , detection limit , repeatability , colloidal gold , chemistry , laccase , chromatography , calibration curve , cyclodextrin , electrode , linear range , nanoparticle , materials science , nuclear chemistry , nanotechnology , organic chemistry , enzyme , antioxidant
This paper describes the synthesis and characterization of gold nanoparticles stabilized in β‐cyclodextrin (AuNP‐CD), which were applied as a platform in the immobilization of laccase (LAC). The AuNP‐CD‐LAC were used in the construction of a new biosensor for rutin determination by square‐wave voltammetry (SWV). Under optimized conditions, the calibration curve showed a linear range for rutin of 0.30 to 2.97 μmol L −1 , with a limit of detection of 0.17 μmol L −1 . The biosensor demonstrated satisfactory repeatability and electrode‐to‐electrode repeatability (with relative standard deviations of 5.6 and 6.0 %, respectively) and good stability. The biosensor was successfully applied in the determination of rutin in different pharmaceutical samples.