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Voltammetric Determination of Tumor Biomarkers for Neuroblastoma (Homovanillic Acid, Vanillylmandelic Acid, and 5‐Hydroxyindole‐3‐acetic Acid) at Screen‐printed Carbon Electrodes
Author(s) -
Makrlíková Anna,
Ktena Evangelia,
Economou Anastasios,
Fischer Jan,
Navrátil Tomáš,
Barek Jiří,
Vyskočil Vlastimil
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600534
Subject(s) - vanillylmandelic acid , homovanillic acid , acetic acid , chemistry , chromatography , differential pulse voltammetry , detection limit , cyclic voltammetry , electrode , electrochemistry , biochemistry , serotonin , receptor
Three tumor biomarkers (homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5‐hydroxyindole‐3‐acetic acid (5‐HIAA)) have been determined by differential pulse voltammetry (DPV) at screen‐printed carbon electrodes (SPCEs). As an optimum medium for all three analytes, Britton‐Robinson buffer (0.04 mol ⋅ L −1 , pH 3.0) was used. Dependencies of the peak current on the concentration of a biomarker were linear in the concentration range from 0.2 to 100 μmol ⋅ L −1 for all the tested compounds, with the limits of quantification 0.8 μmol ⋅ L −1 for homovanillic acid, 0.2 μmol ⋅ L −1 for vanillylmandelic acid, and 0.4 μmol ⋅ L −1 for 5‐hydroxyindole‐3‐acetic acid. Moreover, it was verified that the newly developed methods can be used for simultaneous determination of HVA and VMA in mixture.