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Simultaneous Determination of Peroxide Hydrogen and Ascorbic Acid by Capillary Electrophoresis with Platinum Nanoparticles Modified Micro‐disk Electrode
Author(s) -
Wang J.,
Zhou M.,
Dong R.,
Cong X.,
Zhang R.,
Wang X.
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600407
Subject(s) - ascorbic acid , detection limit , capillary electrophoresis , chemistry , hydrogen peroxide , platinum nanoparticles , platinum , electrode , electrochemistry , linear range , analytical chemistry (journal) , nuclear chemistry , inorganic chemistry , chromatography , catalysis , organic chemistry , food science
Abstract In this study, the first application of a capillary zone electrophoresis‐electrochemical detection (CE‐ECD) method for concurrent determination of hydrogen peroxide (H 2 O 2 ) and ascorbic acid (AA), was developed using the Pt nanoparticles (PtNPs) modified Pt micro‐disk electrode (PtME). The electrocatalytic activity of the modified electrode for H 2 O 2 and AA was characterized by cyclic voltammetry. Under optimized experimental conditions, highly linear calibration plots were observed for both H 2 O 2 and AA, with concentration linear ranges of 0.8 μM to 0.8 mM and 1.0 μM to 0.8 mM. Detection limits of 0.2 μM H 2 O 2 and 0.5 μM AA were determined on the basis of the signal‐to‐noise characteristics (S/N=3) of an electropherogram. Compared with the unmodified PtME, the sensitivity was promoted in that PtNPs/PtME provided an increased effective electrode surface and high catalytic activity toward H 2 O 2 and AA. Using this method, the added H 2 O 2 and AA in Mizone, a kind of functional drink, were detected, and the concentration of AA was found to be 2.33 mM ( n =3). The recovery rates were 95.3 % for H 2 O 2 and 98.7 % for AA. The novel approach provided a wide linear range, low detection limit, good reproducibility and stability. It will provide a new insight into the balance of reactive oxygen species and antioxidant in biological systems.