Premium
Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation
Author(s) -
Issaad Fatima Zohra,
Fernandes Isabel P. G.,
Enache Teodor A.,
Mouats Chabane,
Rodrigues Isaide A.,
OliveiraBrett Ana Maria
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600370
Subject(s) - chemistry , dpph , flavonoid , extraction (chemistry) , antioxidant , differential pulse voltammetry , chromatography , catechin , polyphenol , food science , scavenger , pear , cyclic voltammetry , biochemistry , botany , electrochemistry , biology , electrode
Flavonoids are natural phenolic derivatives that, in low concentration, can provide health benefits by preventing biomolecules (proteins, nucleic acids, lipids, sugars) oxidative damage through free radical mediated reactions. The flavonoids, in selected Mediterranean seasonal fruits: apricot, sour cherry, plum, pomegranate, date, prickly pear (cactus fruit), and nectarine, by RP‐HPLC, coupled with photodiode array and electrochemical detectors, after microwave‐ultrasound assisted extraction, using flavonoid standards, were detected. The total antioxidant capacity in the lyophilized fruit extracts, by differential pulse voltammetry, (electrochemical index‐EI), integrated peak area, and chronoamperometry, was evaluated. In the lyophilized fruit extracts, and the catechin standard, the free radical scavenger efficient concentration (EC 50 ), using DPPH . assay, was determined.