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Voltammetry at a Hanging Mercury Drop Electrode as a Tool for the Study of the Interaction of Double‐stranded DNA with Genotoxic 4‐Nitrobiphenyl
Author(s) -
Horakova Eva,
Barek Jiri,
Vyskocil Vlastimil
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600241
Subject(s) - hanging mercury drop electrode , chemistry , cyclic voltammetry , electrode , voltammetry , dropping mercury electrode , adsorption , differential pulse voltammetry , electrochemistry , mercury (programming language) , analytical chemistry (journal) , chromatography , organic chemistry , computer science , programming language
Differential pulse voltammetry, cyclic voltammetry, alternating current voltammetry, and chronocoulometry at a hanging mercury drop electrode (HMDE) and DNA modified HMDE were used for the study of the interaction of 4‐nitrobiphenyl (4‐NBP) with double‐stranded DNA and for the detection of DNA damage caused by its interaction with 4‐NBP. Moreover, the interaction of DNA with intermediates of the 4‐NBP reduction and with 4‐aminobiphenyl, a metabolite of 4‐NBP, was investigated. The mechanism of the electrochemical reduction of 4‐NBP was investigated using CV, too. It was found that the reduction of 4‐NBP is controlled by both diffusion and adsorption. The rate constant of the reduction and the diffusion coefficient were calculated as well.