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Selective Detection of Dopamine in the Presence of Ascorbic and Uric Acids through its Covalent Immobilization on Gold Mercaptopropionic Acid Self‐assembled Monolayer
Author(s) -
Karimi Shervedani Reza,
Bahrani Sonia,
Samiei Foroushani Marzieh,
Momenbeik Fariborz
Publication year - 2017
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600220
Subject(s) - ascorbic acid , differential pulse voltammetry , detection limit , chemistry , monolayer , covalent bond , carbodiimide , cyclic voltammetry , hydrochloride , self assembled monolayer , electrode , redox , adsorption , uric acid , nuclear chemistry , chromatography , inorganic chemistry , electrochemistry , organic chemistry , biochemistry , food science
A simple, stable and recoverable sensor is developed based on selective and covalent immobilization of dopamine (DA) on the gold (Au) electrode modified by mercaptopropionic acid (MPA) self‐assembled monolayers and then, activated with 1‐ethyl‐3(3‐(dimethylamino)propyl)‐carbodiimide hydrochloride (EDC)/n‐hydroxysuccinimide (NHS) activators. Fabrication steps and analytical application of this modified electrode toward quantitative determination of DA in the presence of ascorbic and uric acids are verified by cyclic and differential pulse voltammetry (CV and DPV) methods in the absence of any redox probe. The oxidation peak current of adsorbed DA changed linearly with the concentration of DA in the range of 0.5 to 600.0 μM with a detection limit of 0.064 μM, and the RSDs varied from 1.4 to 3.5 % for n=3 at each point. Finally, the fabricated sensor is used successfully for electroanalytical determination of DA in human blood plasma and pharmaceutical injection real samples.