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Isotretinoin Oxidation and Electroanalysis in a Pharmaceutical Drug Using a Boron‐doped Diamond Electrode
Author(s) -
Machini W. B. S.,
Enache T. A.,
Jorge S. M. A.,
OliveiraBrett A. M.
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600206
Subject(s) - differential pulse voltammetry , cyclic voltammetry , voltammetry , analytical chemistry (journal) , diffusion , chemistry , diamond , isotretinoin , electrode , materials science , electrochemistry , chromatography , organic chemistry , acne , medicine , thermodynamics , dermatology , physics
Isotretinoin redox behaviour, by voltammetry, at the cathodic pre‐treated boron doped diamond electrode by cyclic, differential pulse and square wave voltammetry, and by spectrophotometry in a wide pH range, was investigated. The isotretinoin oxidation was an irreversible, diffusion‐controlled process, occurred in a three‐step cascade mechanism, and the isotretinoin diffusion coefficient D 13‐cis‐RA =7.35×10 −6 cm 2 s −1 , was calculated. The UV‐Vis isotretinoin maximum absorption was pH‐dependent. The detection limits LOD=0.53 μM, by differential pulse voltammetry, and LOD=0.38 μM, by UV‐Vis spectroscopy, were obtained. The isotretinoin in the pharmaceutical drug Roacutan ® , using differential pulse voltammetry, was determined.