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Label‐free Electrochemical Impedance Detection of Rotavirus Based on Immobilized Antibodies on Gold Sononanoparticles
Author(s) -
Attar Aisha,
Mandli Jihane,
Ennaji My Mustapha,
Amine Aziz
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600179
Subject(s) - detection limit , dielectric spectroscopy , rotavirus , linear range , electrode , colloidal gold , electrochemistry , materials science , chromatography , chemistry , virus , nanotechnology , virology , nanoparticle , biology
Currently, rotaviruses (RVs) are the leading cause of severe childhood gastroenteritis worldwide. This study proposes a sensitive and simple label‐free assay for determining rotaviruses using, for the first time, electrochemical impedance spectroscopy (EIS). The immunosensor is fabricated by self‐assembled monolayers (SAMs) of cysteamine on gold sononanoparticles (AuSNPs) modified glassy carbon electrode. The electron transfer resistance (R et ) of the electrode interface was adopted as a parameter for the detection of rotavirus. Using the proposed immunosensor, a good linear relationship (r 2 =0.993) between impedance change and logarithmic value of rotaviruses in a wide range of 4.6 to 4.6×10 4 PFU mL −1 was found, with a low limit of detection (LOD) 2.3 PFU mL −1 and a total analysis time of 55 min. Working parameters of the immunosensor, such as pH, antibodies concentration and immune‐reaction time, have been investigated and optimized. No obvious interferences from non‐target viruses such as hepatitis A virus (HAV) and enterovirus were found. In addition, the electrochemical impedance immunosensor has exhibited good sensitivity, selectivity and stability.