Electrochemical Biosensor for Naphthalene Acetic Acid in Fruits and Vegetables Based on Lipid Films with Incorporated Auxin‐binding Protein Receptor Using Graphene Electrodes | Zendy

Bratakou Spyridoula | Zendy; Nikoleli GeorgiaParaskevi | Zendy; Siontorou Christina G. | Zendy; Karapetis Stephanos | Zendy; Nikolelis Dimitrios P. | Zendy; Tzamtzis Nikolaos | Zendy

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Electrochemical Biosensor for Naphthalene Acetic Acid in Fruits and Vegetables Based on Lipid Films with Incorporated Auxin‐binding Protein Receptor Using Graphene Electrodes

Author(s) -

Bratakou Spyridoula,

Nikoleli GeorgiaParaskevi,

Siontorou Christina G.,

Karapetis Stephanos,

Nikolelis Dimitrios P.,

Tzamtzis Nikolaos

Publication year - 2016

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.201600152

Subject(s) - biosensor , graphene , potentiometric titration , chemistry , detection limit , selectivity , electrode , linear range , electrochemistry , acetic acid , reproducibility , chromatography , materials science , biochemistry , nanotechnology , catalysis

The present work describes a miniaturized potentiometric naphthalene acetic acid (NAA) sensor on graphene nanosheets with incorporated lipid films. Auxin‐binding protein 1 receptor immobilized on the stabilized lipid films provided adequate selectivity for detection over a wide range of hormone concentrations, fast response time of ca. 5 min, and detection limit of 10 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 56 mV/decade of hormone concentration. The reliability of the biosensor was successfully evaluated using a wide range of NAA‐spiked fruits and vegetables.

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