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Electrochemical Biosensor for Naphthalene Acetic Acid in Fruits and Vegetables Based on Lipid Films with Incorporated Auxin‐binding Protein Receptor Using Graphene Electrodes
Author(s) -
Bratakou Spyridoula,
Nikoleli GeorgiaParaskevi,
Siontorou Christina G.,
Karapetis Stephanos,
Nikolelis Dimitrios P.,
Tzamtzis Nikolaos
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600152
Subject(s) - biosensor , graphene , potentiometric titration , chemistry , detection limit , selectivity , electrode , linear range , electrochemistry , acetic acid , reproducibility , chromatography , materials science , biochemistry , nanotechnology , catalysis
The present work describes a miniaturized potentiometric naphthalene acetic acid (NAA) sensor on graphene nanosheets with incorporated lipid films. Auxin‐binding protein 1 receptor immobilized on the stabilized lipid films provided adequate selectivity for detection over a wide range of hormone concentrations, fast response time of ca. 5 min, and detection limit of 10 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 56 mV/decade of hormone concentration. The reliability of the biosensor was successfully evaluated using a wide range of NAA‐spiked fruits and vegetables.