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Employment of Voltammetry in Studies of Transport Processes across Artificial Phospholipid Membranes
Author(s) -
Šestáková Ivana,
Navrátil Tomáš,
Josypčuk Bohdan
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600135
Subject(s) - chemistry , membrane , ionophore , phospholipid , citric acid , voltammetry , cyclic voltammetry , mercury (programming language) , hanging mercury drop electrode , cadmium , malic acid , mastoparan , chromatography , nuclear chemistry , inorganic chemistry , electrode , electrochemistry , organic chemistry , biochemistry , computer science , programming language , receptor , g protein
This paper reports on voltammetric methods applied in studies of transport processes connected with the occurrence of toxic cadmium in plants. As a means of transport across model of phospholipid membrane (PLM), calcium ionophore (calcimycin), cell‐penetrating peptides (transportan 10 and mastoparan), and finally protoplasts isolated from barley roots and leaves were used. Using calcimycin, different influences of Cd complexation by citric acid or by malic acid as examples of root exudates were documented. For transport of simplest phytochelatin PC 2 , voltammetric methods using formation of Hg complex on hanging mercury drop electrode (HMDE) or of Ag complex on mercury meniscus covered silver solid amalgam electrode (m‐AgSAE) were assessed.