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Boron‐doped Diamond Electrodes Modified with Fe 3 O 4 @Au Magnetic Nanocomposites as Sensitive Platform for Detection of a Cancer Biomarker, Interleukin‐8.
Author(s) -
Braiek Mohamed,
Yang Yi,
Farre Carole,
Chaix Carole,
Bessueille François,
Baraket Abdoullatif,
Errachid Abdelhamid,
Zhang Aidong,
JaffrezicRenault Nicole
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600060
Subject(s) - detection limit , dielectric spectroscopy , cyclic voltammetry , materials science , differential pulse voltammetry , magnetic nanoparticles , electrode , nuclear chemistry , chemistry , amperometry , nanocomposite , analytical chemistry (journal) , electrochemistry , nanoparticle , nanotechnology , chromatography
Interleukin‐8 (IL‐8), a dimeric protein composed of two identical subunits, is overexpressed in different human cancers, among them pancreatic cancers and head and neck cancers. There is a strong demand for monitoring of this biomarker protein for early cancer detection. A new concept of label‐free impedimetric immunosensors for IL‐8 detection is described in this work. A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe 3 O 4 core is synthesized using cysteamine as linker, and characterized using TEM and UV‐Vis spectrometry. After immobilization of these Fe 3 O 4 @Au magnetic nanocomposites on a boron doped diamond (BDD) electrode using a simple magnet, anti‐human IL‐8 monoclonal antibody is linked to the AuNPs, previously functionalized with thioctic acid. The functionalization of the immunosensor was characterized using two electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), performed in phosphate buffer with ferro/ferricyanide as the redox probe. The EIS technique was used for affinity assays: antibody‐Antigen binding. A linear relationship between the increment in the electron transfer resistance (R CT ) and the logarithmic value of IL‐8 concentration was observed between 0.1 pg/mL and 1000 pg/mL. The limit of detection (LOD) was estimated at 0.03 pg/mL.