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MIP‐esterase/Tyrosinase Combinations for Paracetamol and Phenacetin
Author(s) -
Yarman Aysu,
Scheller Frieder W.
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201600042
Subject(s) - chemistry , tyrosinase , resorcinol , aniline , salicylic acid , phenacetin , ascorbic acid , chromatography , amperometry , molecular imprinting , nuclear chemistry , molecularly imprinted polymer , electrochemistry , combinatorial chemistry , catechol , uric acid , electrode , organic chemistry , selectivity , biochemistry , enzyme , catalysis , food science
A new electrochemical MIP sensor for the most frequently used drug paracetamol (PAR) was prepared by electropolymerization of mixtures containing the template molecule and the functional monomers o‐phenylenediamine, resorcinol and aniline. The imprinting factor of 12 reflects the effective target binding to the MIP as compared with the non‐imprinted electropolymer. Combination of the MIP with a nonspecific esterase allows the measurement of phenacetin – another analgesic drug. In the second approach the PAR containing sample solution was pretreated with tyrosinase in order to prevent electrochemical interferences by ascorbic acid and uric acid. Interference‐free indication at a very low electrode potential without fouling of the electrode surface was achieved with the o‐phenylenediamine: resorcinol‐based MIP.