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Applying Mesoporous Silica SBA‐15 in Electrochemical Detection of DNA Hybridization
Author(s) -
Josypčuk Oksana,
Fojta Miroslav,
Daňhel Aleš,
Josypčuk Bohdan
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201501137
Subject(s) - mesoporous silica , electrochemistry , cathodic protection , mesoporous material , covalent bond , dna , dna–dna hybridization , cathodic stripping voltammetry , stripping (fiber) , nanotechnology , materials science , combinatorial chemistry , chemistry , chemical engineering , electrode , organic chemistry , biochemistry , catalysis , composite material , engineering
Mesoporous silica SBA‐15 powder was used as the separation surface for hybridization purposes and it has proved appropriate and effective. By help of a simple procedure any oligodeoxynucleotide (ODN) modified by NH 2 ‐group can be covalently bound at the surface of SiO 2 particles, what significantly extends the capabilities of the experimenter. Use of cathodic stripping voltammetry for electrochemical detection of ODN/DNA hybridization ensures high sensitivity of the analyses. Only ordinary laboratory equipment is needed for the implementation of these experiments. Due to the very small consumption of SBA‐15, commonly sold quantity (5 g) may be sufficient for many years of work.