Premium
Development of an Electrochemical Biosensor for the Rapid Detection of Cholera Toxin Based on Air Stable Lipid Films with Incorporated Ganglioside GM1 Using Graphene Electrodes
Author(s) -
Karapetis Stephanos,
Nikoleli GeorgiaParaskevi,
Siontorou Christina G.,
Nikolelis Dimitrios P.,
Tzamtzis Nikolaos,
Psaroudakis Nikolas
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201501134
Subject(s) - cholera toxin , toxin , biosensor , graphene , ganglioside , detection limit , selectivity , electrode , materials science , potentiometric titration , nanotechnology , chemistry , reproducibility , chromatography , combinatorial chemistry , biochemistry , microbiology and biotechnology , biology , catalysis
The present work describes a miniaturized potentiometric cholera toxin sensor on graphene nanosheets with incorporated lipid films. Ganglioside GM1, the natural cholera toxin receptor, immobilized on the stabilized lipid films, provided adequate selectivity for detection over a wide range of toxin concentrations, fast response time of ca. 5 min, and detection limit of 1 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 60 mV/decade of toxin concentration. The method was implemented and validated in lake water samples. This novel ultrathin film technology is currently adapted to the rapid detection of other toxins that could be used in bioterrorism.