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Electrochemical Biosensor Utilizing Supramolecular Association of Enzyme on Sol−gel Matrix Embedded Gold Nanoparticles Supported Reduced Graphene Oxide−cyclodextrin Nanocomposite
Author(s) -
Manivannan Shanmugam,
Kim Kyuwon
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201501104
Subject(s) - graphene , nanocomposite , supramolecular chemistry , biosensor , materials science , nanoparticle , conjugate , hydroquinone , cyclodextrin , combinatorial chemistry , chemistry , nanotechnology , organic chemistry , mathematical analysis , mathematics , crystal structure
Abstract Herein we present β‐cyclodextrin (CD)‐functionalized reduced graphene oxide (RGO) nanosheets supported on silicate sol‐gel matrix‐embedded gold nanoparticles (Au NPs) modified electrode as a new affinity binding nanocomposite. The modified electrode is fabricated through layer‐by‐layer drop casting followed by immobilization of chemically modified enzyme conjugate (horse radish peroxidase (HRP)−adamantane carboxylic acid (ADA)). This affinity system is based on the supramolecular association between CDs and HRP−ADA and is mimicking the biological avidin−biotin interactions. CDs‐functionalized RGO (RGO−CD) functions as a macrocyclic host to form stable supramolecular inclusion complexes with enzyme conjugate. Besides Au NPs improve the interfacial interaction with RGO−CD nanosheets, and thus exhibit synergistic electrocatalytic effect toward H 2 O 2 reduction in the presence of 1 mM hydroquinone.