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A Sensitive Biosensor for Determination of Cu 2+ by One‐step Electrodeposition
Author(s) -
Tian Rong,
Chen Xiaojun,
Liu Dejin,
Yao Cheng
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201501070
Subject(s) - biosensor , detection limit , nanorod , cyclic voltammetry , materials science , prussian blue , dielectric spectroscopy , electrochemistry , scanning electron microscope , transmission electron microscopy , copper , electrode , analytical chemistry (journal) , linear range , nanotechnology , nuclear chemistry , chemistry , chromatography , composite material , metallurgy
A simple one‐step electrodeposition method is described to fabricate three dimensional ordered macroporous chitosan−prussian blue−single walled carbon nanotubes (3DOM CS−PB−SWCNTs) film onto the gold electrode surface to fabricate a copper ions (Cu 2+ )‐specific DNAzyme biosensor. The new sensing strategy for sensitive and selective detection of Cu 2+ was based on Au nanorods (AuNRs) as signal amplification labels. The electrochemical signal of glucose increased with the concentration of Cu 2+ increasing. The morphologies and electrochemistry of the composites were investigated by using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical techniques including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) and so on. Linear correlations of copper ion concentration were obtained in the range from 10 −18 M to 10 −5 M, achieving with a limit of detection of 10 −19 M (S/N=3). Parameters affecting the biosensor response such as temperature, the cleavage time and the time of hybridization were optimized. This biosensor showed a wide range, low detection limit, good reproducibility and high stability. Additionally, these striking properties endow the biosensor with a great promise for analytical applications.