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Construction of 5‐amino‐1,10‐phenanthroline‐Fe(II) Nanostructures on Glassy Carbon Electrode: Simultaneous and Selective Determination of Purine and Pyrimidine DNA Bases
Author(s) -
Shervedani Reza Karimi,
Dehaghi Sedigheh Bagheri,
Foroushani Marzieh Samiei
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500580
Subject(s) - guanine , thymine , pyrimidine , nucleobase , electrode , cytosine , dna , phenanthroline , electrochemistry , chemistry , purine , carbon paste electrode , base pair , inorganic chemistry , stereochemistry , cyclic voltammetry , organic chemistry , nucleotide , biochemistry , gene , enzyme
5‐amino‐1,10‐phenanthroline‐Fe(II) complex is immobilized onto GC electrode and used for determination of DNA bases. Modifications are traced by electrochemical methods. All DNA bases are electroactive on the modified electrode. The I p s increased linearly with increase of DNA bases concentration. A wide response range was observed for each base (∼4 orders for guanine (GA) and adenine (A); and ∼2.5 orders for thymine (T) and cytosine (C)) with DLs of 0.15, 4.44, 133.0 and 230.0 nM, respectively. The electrode was applied for determination of calf‐thymus DNA bases. The value obtained for [(GA+C)/(A+T)], 0.78, is in good agreement with standard value, 0.77.