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An Electrochemical Biosensor with Uricase Immobilized on Functionalized Gold Coated Copper Wire Electrode for Urinary Uric Acid Assay
Author(s) -
Cheng Cheanyeh,
Kao ChiYing
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500539
Subject(s) - uric acid , electrode , copper , biosensor , electrochemistry , detection limit , chemistry , urine , surface modification , materials science , nuclear chemistry , inorganic chemistry , chromatography , biochemistry , organic chemistry
Abstract A new second generation uricase electrode for urinary uric acid determination has been developed by chemically binding both uricase and redox mediator to inexpensive copper wire through simple electrodeposition of gold on copper surface and subsequent functionalization of the gold with L‐methionine. During a 209‐day testing period, the overall electrode performance exhibits in average a low oxidation potential of 0.33 V, a response time of 5 s, a widest linear calibration concentration range (0–2.38 mM, r 2 >0.9952), a sensitivity of 50 μA mM −1 , and a detection limit of 2.4 μM. The measurement accuracy and precision for the determination of uric acid in human urine specimens were 85.6–95.5 % and 0.3–2.4 %, respectively. The developed uricase electrode is potential for clinical applications.