Premium
A Galactose Oxidase Biosensor Based on Graphene Composite Film for the Determination of Galactose and Dihydroxyacetone
Author(s) -
Xie Jia,
Chen Chao,
Zhou Yuanqing,
Fei Junjie,
Ding Yonglan,
Zhao Jia
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500486
Subject(s) - biosensor , galactose oxidase , cyclic voltammetry , amperometry , graphene , glucose oxidase , materials science , nanotechnology , galactose , redox , chemistry , chemical engineering , analytical chemistry (journal) , electrochemistry , chromatography , inorganic chemistry , organic chemistry , electrode , engineering
A novel amperometric biosensor was developed by immobilizing galactose oxidase (GAox) on poly (L‐lactide) stabilized gold nanoparticles (Au@PLLA) modified graphene nano‐composite film (RGO‐Au@PLLA/GAox). The direct electron transfer (DET) of GAox was achieved. Cyclic voltammetry of the GAox in the RGO‐Au@PLLA film revealed a pair of well‐defined and quasireversible peaks at about −0.137 V (vs. SCE), which is characterized of the Cu(II)/Cu(I) redox couple for the galactose oxidase. Amperometric response was measured as a function for the determination of galactose (GA) and dihydroxyacetone (DHA). The constructed biosensor also possesses good stability, reproducibility and anti‐interference ability.