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Enhanced Biosensing of Bisphenol A Using a Nanointerface Based on Tyrosinase/Reduced Graphene Oxides Functionalized with Ionic Liquid
Author(s) -
Li Rui,
Wang Yanying,
Deng Yinghua,
Liu Guishen,
Hou Xiaodong,
Huang Yina,
Li Chunya
Publication year - 2016
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500448
Subject(s) - ionic liquid , graphene , bisphenol a , dielectric spectroscopy , detection limit , pyridinium , tyrosinase , biosensor , oxide , glassy carbon , materials science , nuclear chemistry , electrochemistry , tetrafluoroborate , inorganic chemistry , chemistry , electrode , nanotechnology , organic chemistry , cyclic voltammetry , chromatography , epoxy , enzyme , catalysis
Reduced graphene oxide (rGO) nanosheets functionalized with 1,3‐di(4‐amino‐1‐pyridinium) propane tetrafluoroborate ionic liquid (DAPPT) was successfully synthesized and fully characterized. DAPPT‐rGO nanosheets were coated to a glassy carbon electrode (GCE) surface to immobilize tyrosinase (Tyr). The Tyr‐DAPPT‐rGO/GCE was characterized with scanning electronic microscopy and electrochemical impedance spectroscopy. The biosensor shows a linear response to bisphenol A (BPA) in the concentration range of 1.0×10 −9 ∼3.8×10 −5  mol L −1 . The detection limit was calculated to be 3.5×10 −10  mol L −1 (S/N=3). The practical application was demonstrated by determining BPA leaching from commercial plastic drinking bottles.

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