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Voltammetric Behaviour of 7‐Methylguanine Using Screen‐printed Graphite Electrodes: towards a Guanine Methylation Electrochemical Sensor
Author(s) -
Brotons Ariadna,
Sanjuan Ignacio,
Banks Craig E.,
VidalIglesias Francisco José,
SollaGullón José,
Iniesta Jesús
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500263
Subject(s) - guanine , electrochemistry , cyclic voltammetry , electrode , methylation , graphite , electrochemical gas sensor , epigenetics , chemistry , dna methylation , dna , materials science , biochemistry , organic chemistry , gene , nucleotide , gene expression
The determination of 7‐methylguanine (7mG) is an interesting analytical target since its quantification can be correlated with the methylation of cytosine which is one of the most relevant epigenetic modification of DNA. In this work, screen‐printed graphite electrodes were used to study the electrochemical characterization of 7mG by cyclic and square wave voltammetry. 7mG concentration and pH were examined, as well as the repeatability of the measurements. The electrochemical response of 7mG in the presence of guanine and adenine was also investigated. Results aim to the future development of an electrochemical sensor for the methylation degree in DNA.