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Biosensing Applications of V 2 O 5 ‐CeO 2 Mesoporous Silica
Author(s) -
Luo Xi,
Shu Hao,
Wan Qijin,
Wang Zhaohao,
Yang Nianjun
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500250
Subject(s) - detection limit , cerium oxide , cyclic voltammetry , biosensor , electrode , mesoporous material , mesoporous silica , cerium , materials science , reaction rate constant , pentoxide , catalysis , vanadium , inorganic chemistry , chemistry , nuclear chemistry , nanotechnology , electrochemistry , chromatography , kinetics , organic chemistry , quantum mechanics , physics
Mesoporous silica has been functionalized with vanadium pentoxide (V 2 O 5 ) and cerium oxide (CeO 2 ). It was then immobilized with biocatalyst horseradish (HRP) to realize highly sensitive detection of H 2 O 2 . The electrode was characterized with SEM, cyclic voltammetry, and impedance measurements. A well‐defined peak for HRP reduction was noticed at about −0.31 V with an apparent heterogeneous electron transfer rate constant of 1.01 s −1 . The catalytic reduction of H 2 O 2 was achieved. The Michaelis constant was calculated to be 0.018 mM. On this electrode, H 2 O 2 was detectable in the range of 20 to 180 µM and the detection limit was 3.0 µM.