Intercalation of Proflavine in ssDNA Aptamers: Effect on Binding of the Specific Target Chloramphenicol | Zendy

Pilehvar Sanaz | Zendy; Jambrec Daliborka | Zendy; Gebala Magdalena | Zendy; Schuhmann Wolfgang | Zendy; De Wael Karolien | Zendy

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Intercalation of Proflavine in ssDNA Aptamers: Effect on Binding of the Specific Target Chloramphenicol

Author(s) -

Pilehvar Sanaz,

Jambrec Daliborka,

Gebala Magdalena,

Schuhmann Wolfgang,

De Wael Karolien

Publication year - 2015

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.201500192

Subject(s) - aptamer , proflavine , intercalation (chemistry) , chemistry , chloramphenicol , biophysics , molecule , electrode , crystallography , dna , biochemistry , inorganic chemistry , microbiology and biotechnology , biology , antibiotics , organic chemistry

The structural modification of ssDNA‐based aptamers upon specific binding of its target molecule leads to changes of the charge‐transfer resistance ( R ct ) of a negatively‐charged free‐diffusing redox probe. The aptamer adopts a structure due to self‐hybridization which is stabilized using profalvine as intercalator. The pre‐organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the R ct values. Pre‐incubation of the aptamer‐modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP‐dependent variation of the R ct values.

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