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Intercalation of Proflavine in ssDNA Aptamers: Effect on Binding of the Specific Target Chloramphenicol
Author(s) -
Pilehvar Sanaz,
Jambrec Daliborka,
Gebala Magdalena,
Schuhmann Wolfgang,
De Wael Karolien
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500192
Subject(s) - aptamer , proflavine , intercalation (chemistry) , chemistry , chloramphenicol , biophysics , molecule , electrode , crystallography , dna , biochemistry , inorganic chemistry , microbiology and biotechnology , biology , antibiotics , organic chemistry
The structural modification of ssDNA‐based aptamers upon specific binding of its target molecule leads to changes of the charge‐transfer resistance ( R ct ) of a negatively‐charged free‐diffusing redox probe. The aptamer adopts a structure due to self‐hybridization which is stabilized using profalvine as intercalator. The pre‐organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the R ct values. Pre‐incubation of the aptamer‐modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP‐dependent variation of the R ct values.