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Acidic and Basic Functionalized Carbon Nanomaterials as Electrical Bridges in Enzyme Loaded Chitosan/Poly(styrene sulfonate) Self‐Assembled Layer‐by‐Layer Glucose Biosensors
Author(s) -
David Melinda,
Barsan Madalina M.,
Florescu Monica,
Brett Christopher M. A.
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500171
Subject(s) - glucose oxidase , biosensor , cyclic voltammetry , chitosan , sulfonate , amperometry , nanomaterials , graphene , carbon nanotube , layer by layer , electrode , materials science , styrene , dielectric spectroscopy , electrochemistry , immobilized enzyme , chemical engineering , conductive polymer , chemistry , nanotechnology , polymer , copolymer , layer (electronics) , organic chemistry , enzyme , engineering , sodium
Glucose oxidase (GOx) was incorporated in a self‐assembled multilayer modified gold electrode, based on electrostatic interaction between positively charged chitosan polymer, containing GOx, and negatively charged poly(styrene sulfonate). Good electronic communication between electrode and enzyme was ensured by carbon nanomaterials (CN), graphene (G) or carbon nanotubes (CNT) functionalised in either HNO 3 or KOH, immobilized together with the enzyme. Cyclic voltammetry and electrochemical impedance spectroscopy enabled identification of the glucose biosensor assemblies with the best functionalized CN, KOH_G and HNO 3 _CNT, confirmed by fixed potential amperometry at −0.3 V vs. Ag/AgCl, these glucose biosensors exhibiting the highest sensitivities and lowest detection limits.