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A Highly Sensitive Electrochemical Immunosensor for Fumonisin B 1 Detection in Corn Using Single‐Walled Carbon Nanotubes/Chitosan
Author(s) -
Yang Xianxian,
Zhou Xipeng,
Zhang Xian,
Qing Ying,
Luo Mei,
Liu Xiao,
Li Chaorui,
Li Yingli,
Xia Huiming,
Qiu Jingfu
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500169
Subject(s) - detection limit , carbon nanotube , fumonisin , fumonisin b1 , chitosan , chemistry , electrochemistry , chromatography , nuclear chemistry , covalent bond , electrode , materials science , nanotechnology , mycotoxin , organic chemistry , food science
A sensitive electrochemical immunosensor was developed for detecting fumonisin B 1 (FB 1 ) in corn using the single‐walled carbon nanotubes/chitosan. The detection mechanism of immunosensor was based on an indirect competitive binding to a fixed amount of anti‐FB 1 between free FB 1 and FB 1 ‐bovine serum albumin, which was conjugated on covalently functionalized nanotubes/chitosan laid on the glass carbon electrode. The anti‐rabbit immunoglobulin G secondary antibody labeled with alkaline phosphatase was then bound to the electrode surface through reactisubstrate α‐naphthyl phosphate, which produced electrochemical signal. Under optimized conditions, this method could detect FB 1 from 0.01 to 1000 ng mL −1 with a detection limit of 2 pg mL −1 . This is well below the detection limit required from European Union legislation, 2–4 mg L −1 . Moreover, good recoveries were obtained for the detection of spiked corn samples and actual corn samples. As the method has good sensitivity and recovery for detecting FB 1 , it is a practical detection method.