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An Online Electrochemical System for Continuous Measurement of Glutamate with Signal Amplification by Enzymatic Substrate Cycling
Author(s) -
Zhang Zipin,
Xiao Tongfang,
Hao Jie,
Yu Ping,
Ohsaka Takeo,
Mao Lanqun
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500122
Subject(s) - microdialysis , glutamate receptor , substrate (aquarium) , biosensor , electrochemistry , materials science , biophysics , chemistry , nanotechnology , biomedical engineering , electrode , neuroscience , central nervous system , biochemistry , biology , medicine , ecology , receptor
This study demonstrates a new online electrochemical system (OECS) for continuous monitoring of glutamate by efficiently integrating in vivo microdialysis and selective electrochemical detection with an enhanced sensitivity through enzymatic substrate cycling. We find that the involvement of enzymatic substrate cycling in the biosensing scheme remarkably increases the sensitivity of the OECS toward the measurements of glutamate. Moreover, the OECS demonstrated here is stable, reproducible, and selective and could thus be potentially useful for continuous monitoring of glutamate release in central nervous system. This study essentially offers a new approach to the continuous measurements of cerebral glutamate release, which is believed to find interesting applications in understanding the molecular basis underlying brain functions.