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A Layer‐by‐Layer Biosensing Architecture Based on Polyamidoamine Dendrimer and Carboxymethylcellulose‐Modified Graphene Oxide
Author(s) -
Borisova Boryana,
Ramos Javier,
Díez Paula,
Sánchez Alfredo,
Parrado Concepción,
Araque Elena,
Villalonga Reynaldo,
Pingarrón José M.
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500098
Subject(s) - biosensor , dendrimer , graphene , amperometry , glutaraldehyde , detection limit , nanomaterials , materials science , ethylenediamine , electrode , catechol , layer by layer , oxide , nanotechnology , layer (electronics) , electrochemistry , chemical engineering , chemistry , inorganic chemistry , polymer chemistry , chromatography , organic chemistry , engineering
A novel nanostructured architecture for the construction of electrochemical enzyme biosensors is here described. It implies the electrostatic layer‐by‐layer assembly of four‐generation ethylenediamine core polyamidoamine G‐4 dendrimers on glassy carbon electrodes coated with a graphene oxide‐carboxymethylcellulose hybrid nanomaterial. This modified surface was further employed for the covalent immobilization of the model enzyme tyrosinase through a glutaraldehyde‐mediated cross‐linking. The prepared enzyme electrode allowed the amperometric detection of catechol in the 2–400 nM range. The biosensor showed excellent analytical performance with high sensitivity of 6.3 A/M and low detection limit of 0.9 nM. The enzyme electrode retained over 93 % of the initial activity after 40 days at 4 °C.