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Potential of Gelatin‐Zinc Oxide Nanocomposite as Ascorbic Acid Sensor
Author(s) -
Rawat Kamla,
Sharma Anshu,
Solanki Pratima R.,
Bohidar H. B.
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201500090
Subject(s) - ascorbic acid , nanocomposite , zinc , gelatin , indium tin oxide , detection limit , nuclear chemistry , materials science , fourier transform infrared spectroscopy , oxide , electrode , chemistry , chemical engineering , nanotechnology , chromatography , metallurgy , layer (electronics) , organic chemistry , food science , engineering
We report on the studies relating to fabrication of gelatin B (GB) and zinc oxide (ZnO) based nanocomposite (GB‐ZnO) film deposited on indium‐tin‐oxide (ITO) glass plate, and used for the immobilization of ascorbate oxidase (AsOx) which was further used for ascorbic acid (AA) detection. The structural and morphological studies of GB‐ZnO, and AsOx/GB‐ZnO/ITO bioelectrodes were carried out using XRD, SEM and FTIR techniques. This bioelectrode showed a broad range of linearity (5–500 mg/dL), low detection limit (1 mg/dL), higher sensitivity (0.106 µA mg/dLcm −2 ) and low value of the apparent MichaelisMenten constant ( K m app =0.35 mg/dL) for AA. Efforts are being made to utilize this electrode for sensing AA in real samples in a bid to develop a strip based sensor.