z-logo
Premium
Faradic Peaks Enhanced by Carbon Nanotubes in Microsomal Cytochrome P450 Electrodes
Author(s) -
BajRossi C.,
Müller C.,
von Mandach U.,
De Micheli G.,
Carrara S.
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400726
Subject(s) - microsome , carbon nanotube , cytochrome p450 , substrate (aquarium) , cyclic voltammetry , chemistry , cytochrome , electrode , adsorption , materials science , electrochemistry , nanotechnology , organic chemistry , enzyme , oceanography , geology
In this work we present an investigation on the behavior of microsomes containing human cytochrome P450 in cyclic voltammetry for drug detection. The microsomes are adsorbed on the surface of multi‐walled carbon nanotubes by drop‐casting. We demonstrate that the hydrophobic and highly electroactive surface of multi‐walled carbon nanotubes enables to distinguish more clearly the contributions in reduction peak current attributed to the enzymatic components of microsomes. Voltammetric measurements were performed under several experimental conditions with two cytochrome P450‐isoforms, 1A2 and 3A4. We show that the reduction current for the component of cytochrome P450‐microsome linearly increases in the presence of a substrate.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here