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Galactose Oxidase/Prussian Blue Based Biosensors
Author(s) -
Charmantray Franck,
Touisni Nadia,
Hecquet Laurence,
Noguer Thierry,
Mousty Christine
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400720
Subject(s) - prussian blue , biosensor , amperometry , glucose oxidase , electrode , hydrogen peroxide , galactose oxidase , materials science , microelectrode , nanotechnology , chemistry , galactose , electrochemistry , organic chemistry
This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly‐( O ‐phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag‐AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx‐TK biosensor.