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Label‐Free Electrochemical Aptasensor for Determination of Chloramphenicol Based on Gold Nanocubes‐Modified Screen‐Printed Gold Electrode
Author(s) -
Bagheri Hashkavayi Ayemeh,
Bakhsh Raoof Jahan,
Ojani Reza,
Hamidi Asl Ezat
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400718
Subject(s) - detection limit , electrode , aptamer , electrochemistry , dielectric spectroscopy , materials science , calibration curve , cyclic voltammetry , voltammetry , analytical chemistry (journal) , colloidal gold , chemistry , chromatography , nanotechnology , nanoparticle , genetics , biology
An ultrasensitive label‐free electrochemical aptasensor was developed for selective detection of chloramphenicol (CAP). The aptasensor was made using screen‐printed gold electrode modified with synthesized gold nanocube/cysteine. The interactions of CAP with aptamer were studied by cyclic voltammetry, square wave voltammetry (SWV) and electrochemical impedance spectroscopy. Under optimized conditions, two linear calibration curves were obtained for CAP determination using SWV technique, from 0.03 to 0.10 µM and 0.25–6.0 µM with a detection limit of 4.0 nM. The aptasensor has the advantages of good selectivity and stability and applied to the determination of CAP in human blood serum sample.