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Dopamine Electroanalysis Using Electrochemical Biosensors Prepared by a Sinusoidal Voltages Method
Author(s) -
Lupu Stelian,
Lete Cecilia,
Javier del Campo Francisco
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400680
Subject(s) - microelectrode , biosensor , detection limit , amperometry , materials science , electrode , biocomposite , tyrosinase , nanotechnology , electrochemistry , chemistry , chromatography , enzyme , composite number , composite material , organic chemistry
A biocomposite material of tyrosinase and poly(3,4‐ethylenedioxythiophene) was electrodeposited onto gold (Au) disk microelectrode arrays and Au interdigitated microband electrode arrays (IDEs) by using a combination of sinusoidal voltages and microgravimetric method to construct amperometric biosensors for dopamine (DA). The main advantage of this new strategy consists in a reliable enzyme immobilization and in additional information related to the electropolymerization process. The determination of DA was successfully achieved in pharmaceutical formulations. The best analytical performances, in terms of the lowest limit of detection of 6×10 −7 M DA and highest recovery and stability, were obtained for IDEs based biosensor.