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Electrochemical Immunosensor for the Determination of Total Ghrelin Hormone in Saliva
Author(s) -
MartínezGarcía Gonzalo,
Serafín Verónica,
Agüí Lourdes,
YáñezSedeño Paloma,
Pingarrón José M.
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400662
Subject(s) - chemistry , ghrelin , chromatography , immunoassay , alkaline phosphatase , linear range , streptavidin , conjugate , biotinylation , detection limit , biotin , biochemistry , receptor , enzyme , mathematical analysis , mathematics , antibody , immunology , biology
An electrochemical immunosensor for ghrelin (GHRL) determination in saliva is reported. Anti‐GHRL was immobilized onto Protein G‐magnetic beads and a competitive immunoassay involving biotinylated GHRL and alkaline phosphatase‐streptavidin was implemented. Once conjugate was magnetically captured on a screen‐printed carbon electrode, GHRL quantization was accomplished by DPV of 1‐naphtol formed upon addition of 1‐naphtyl phosphate. A linear range between 10 −3 and 10 3 ng/mL GHRL, and a LOD of 7 pg/mL, much smaller than those from commercial ELISA kits, were found. The usefulness of the immunosensor was demonstrated by analyzing human saliva spiked with GHRL at 0.01, 0.1, 1 and 10 ng/mL.