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A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles
Author(s) -
HamidiAsl Ezat,
Raoof Jahan Bakhsh,
Hejazi Mohammad Saeid,
Sharifi Simin,
Golabi Seyed Mahdi,
Palchetti Ilaria,
Mascini Marco
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400660
Subject(s) - biotinylation , microbiology and biotechnology , dna , point mutation , biosensor , polymerase chain reaction , mutant , mutation , microfluidics , gene , dna sequencing , streptavidin , chemistry , biology , nanotechnology , materials science , biotin , biochemistry
The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.