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Novel Amperometric Tri‐Enzyme Biosensor for Lysine Determination in Pharmaceutical Products and Food Samples
Author(s) -
Bóka Beáta,
Korózs Marietta,
Nánási Márta,
Adányi Nóra
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400600
Subject(s) - biosensor , amperometry , chromatography , chemistry , immobilized enzyme , horseradish peroxidase , lysine , peroxidase , linear range , flow injection analysis , enzyme , biochemistry , detection limit , electrode , amino acid , electrochemistry
An enzyme‐based amperometric biosensor was developed for the determination of the lysine content of pharmaceutical products, food and feed samples. Lysine decarboxylase (EC 4.1.1.18) from Bacterium cadaveris was coupled with diamine oxidase (EC 1.4.3.6) from Pisum sativum and horseradish peroxidase (EC 1.11.1.7). The enzymes were co‐immobilized on a graphite electrode with an Os polymer. The biosensor worked in flow injection analysis system (FIA). Optimal working conditions were pH 7.0 and −50 mV potential vs. Ag/AgCl. The linear measuring range was 0.005–0.500 mM. Different pharmaceutical products and feed samples were analysed by the lysine biosensor and by HPLC method for validation.