Premium
Electrochemical Biosensors Based on Native DNA and Nanosized Mediator for the Detection of Anthracycline Preparations
Author(s) -
Evtugyn Gennady,
Porfireva Anna,
Stepanova Veronika,
Budnikov Herman
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400564
Subject(s) - daunorubicin , idarubicin , doxorubicin , biosensor , dna , anthracycline , electrochemistry , graphene , electrode , materials science , chemistry , nanotechnology , biochemistry , medicine , surgery , complete remission , chemotherapy , cancer , leukemia , breast cancer
A novel electrochemical DNA‐sensor based on glassy carbon electrode (GCE) modified with electropolymerized Neutral red (NR) and polycarboxylated thiacax[4]arene with covalently attached mediator and electrostatically adsorbed DNA was developed for sensitive detection of anthracycline preparations. Intercalation of doxorubicin, daunorubicin and idarubicin into DNA increases the charge transfer resistance and decreases the electron exchange resulted in decay of the cathodic peak of NR reduction. The DNA‐sensor developed makes it possible to determine down to 0.05 nM doxorubicin, o.1 nM doxorubicin and 0.5 nM idarubicin. The DNA‐sensor was tested on detection of doxorubicin in pharmaceuticals and artificial blood plasma with 95–100 % recovery.