Premium
Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water‐Ethanol
Author(s) -
Cincotto Fernando H.,
Canevari Thiago C.,
Machado Sérgio A. S.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400451
Subject(s) - ergocalciferol , glassy carbon , chemistry , electrochemistry , detection limit , solvent , electrolyte , supporting electrolyte , inorganic chemistry , vitamin , lithium perchlorate , electrode , ethanol , cholecalciferol , chromatography , cyclic voltammetry , organic chemistry , biochemistry
This paper describes the electrochemical determination of vitamin D 2 (ergocalciferol) and D 3 (cholecalciferol) in mixed organic/water solvent, using a glassy carbon electrode (GCE). The mixing ratio of organic/water solvent has an important influence on the electrocatalytic response of D vitamins on the surface of the glassy carbon electrode. Well‐defined peaks for Vitamin D 2 and D 3 were observed in a 40 % ethanol/60 % water solution with lithium perchlorate as the support electrolyte. This study demonstrated that the glassy carbon electrode is highly sensitive for the determination of vitamin D 2 and D 3 , with a limit of detection of 0.13 and 0.118 µmol L −1 , respectively. No significant interference was seen for vitamins A, E and K in the detection of vitamin D.