Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water‐Ethanol | Zendy

Cincotto Fernando H. | Zendy; Canevari Thiago C. | Zendy; Machado Sérgio A. S. | Zendy

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Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water‐Ethanol

Author(s) -

Cincotto Fernando H.,

Canevari Thiago C.,

Machado Sérgio A. S.

Publication year - 2014

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.201400451

Subject(s) - ergocalciferol , glassy carbon , chemistry , electrochemistry , detection limit , solvent , electrolyte , supporting electrolyte , inorganic chemistry , vitamin , lithium perchlorate , electrode , ethanol , cholecalciferol , chromatography , cyclic voltammetry , organic chemistry , biochemistry

This paper describes the electrochemical determination of vitamin D 2 (ergocalciferol) and D 3 (cholecalciferol) in mixed organic/water solvent, using a glassy carbon electrode (GCE). The mixing ratio of organic/water solvent has an important influence on the electrocatalytic response of D vitamins on the surface of the glassy carbon electrode. Well‐defined peaks for Vitamin D 2 and D 3 were observed in a 40 % ethanol/60 % water solution with lithium perchlorate as the support electrolyte. This study demonstrated that the glassy carbon electrode is highly sensitive for the determination of vitamin D 2 and D 3 , with a limit of detection of 0.13 and 0.118 µmol L −1 , respectively. No significant interference was seen for vitamins A, E and K in the detection of vitamin D.

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