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Adsorptive Transfer Stripping for Quick Electrochemical Determination of microRNAs in Total RNA Samples
Author(s) -
Bartosik M.,
Hrstka R.,
Palecek E.,
Vojtesek B.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400449
Subject(s) - microrna , chemistry , rna , stripping (fiber) , electrochemistry , adsorption , chromatography , electrode , drop (telecommunication) , gene , biochemistry , materials science , organic chemistry , composite material , telecommunications , computer science
Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead‐immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter‐sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500‐fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules.