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Impedimetric DNA‐Based Biosensor for Silver Ions Detection with Hemin/G‐Quadruplex Nanowire as Enhancer
Author(s) -
Liu Guangpeng,
Yuan Yali,
Wei Shiqiang,
Zhang Dujian
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400439
Subject(s) - hemin , biosensor , detection limit , dielectric spectroscopy , g quadruplex , chemistry , dna , aptamer , cytosine , deoxyribozyme , ion , electrochemistry , materials science , electrode , microbiology and biotechnology , heme , biochemistry , chromatography , enzyme , biology , organic chemistry
Abstract A DNA‐based biosensor was reported for detection of silver ions (Ag + ) by electrochemical impedance spectroscopy (EIS) with [Fe(CN) 6 ] 4−/3− as redox probe and hybridization chain reaction (HCR) induced hemin/G‐quadruplex nanowire as enhanced label. In the present of target Ag + , Ag + interacted with cytosine‐cytosine (CC) mismatch to form the stable CAg + C complex with the aim of immobilizing the primer DNA on electrode, which thus triggered the HCR to form inert hemin/G‐quadruplex nanowire with an amplified EIS signal. As a result, the DNA biosensor showed a high sensitivity with the concentration range spanning from 0.1 nM to 100 µM and a detection limit of 0.05 nM.