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Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks
Author(s) -
Canali Chiara,
Mohanty Soumyaranjan,
Heiskanen Arto,
Muhammad Haseena Bashir,
Martinsen Ørjan Grøttem,
Dufva Martin,
Wolff Anders,
Emnéus Jenny
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400413
Subject(s) - dielectric spectroscopy , polydimethylsiloxane , porosity , electrical impedance , materials science , scaffold , characterization (materials science) , channel (broadcasting) , biomedical engineering , electrochemistry , analytical chemistry (journal) , nanotechnology , chemistry , composite material , electrode , computer science , chromatography , telecommunications , electrical engineering , engineering
We present the application of electrochemical impedance spectroscopy (EIS) as a method for discriminating between different polydimethylsiloxane (PDMS) scaffolds for three‐dimensional (3D) cell cultures. The validity of EIS characterisation for scaffolds having different degree of porosity (networks of structured or random channels) is discussed in relation to Archie’s law. Guidelines for EIS analysis are presented and demonstrated to provide porosity information in physiological buffer that agrees well with a more conventional weight‐based approach. We also propose frequency ranges that may serve as means of single‐frequency measurements for fast scaffold characterization combined with in vitro monitoring of 3D cell cultures.