Premium
Sensitive Phenol Detection Using Tyrosinase‐Based Phenol Oxidation Combined with Redox Cycling of Catechol
Author(s) -
Noh Soodong,
Yang Haesik
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400383
Subject(s) - catechol , tcep , chemistry , phenol , redox , hydroquinone , benzoquinone , electrochemistry , tyrosinase , detection limit , phosphine , catechol oxidase , inorganic chemistry , nuclear chemistry , photochemistry , electrode , organic chemistry , chromatography , enzyme , catalysis , peroxidase , polyphenol oxidase
This paper reports sensitive phenol detection using (i) tyrosinase (Tyr)‐based oxidation of phenol to catechol, combined with (ii) electrochemical‐chemical‐chemical (ECC) redox cycling involving Ru(NH 3 ) 6 3+ , catechol, and tris(2‐carboxyethyl)phosphine (TCEP). Phenol is converted into catechol by Tyr in the presence of dissolved O 2 . Catechol then reacts with Ru(NH 3 ) 6 3+ , generating o ‐benzoquinone and Ru(NH 3 ) 6 2+ . o ‐Benzoquinone is reduced back to catechol by TCEP, and Ru(NH 3 ) 6 2+ is accumulated over the course of the incubation. When Ru(NH 3 ) 6 2+ is electrochemically oxidized to Ru(NH 3 ) 6 3+ , ECC redox cycling occurs. For simple phenol detection, bare ITO electrodes are used without modifying the electrodes with Tyr. The detection limit for phenol in tap water using Tyr‐based oxidation combined with ECC redox cycling is ca. 10 −9 M, while that using only Tyr‐based oxidation is ca. 10 −7 M.