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Label‐Free Impedance Aptasensor for Major Peanut Allergen Ara h 1
Author(s) -
Trashin Stanislav,
de Jong Mats,
Breugelmans Tom,
Pilehvar Sanaz,
De Wael Karolien
Publication year - 2015
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400365
Subject(s) - aptamer , dielectric spectroscopy , chemistry , chromatography , electrode , allergen , electrochemistry , biology , microbiology and biotechnology , immunology , allergy
The peanut allergen Ara h 1 belonging to the vicilin family of seed storage proteins is known to be a suitable marker for peanut detection in foods. In the present work, we suggest an electrochemical detection of Ara h 1 using a recently selected 80‐base DNA aptamer. The detection strategy relies on insulation effect of a bulky protein captured by an immobilized aptamer. The electrodes were modified by the aptamer and characterized using electrochemical impedance spectroscopy (EIS). The aptamer surface density and analytical parameters of the calibration curves for Ara h 1 were compared with the system prepared with thrombin binding aptamer (TBA) and operated in the same conditions.