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Electrochemical Detection of Activated Protein C Using an Aptasensor Based on PAMAM Dendrimer Modified Pencil Graphite Electrodes
Author(s) -
Erdem Arzum,
Congur Gulsah,
Mese Fehmi
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400354
Subject(s) - dendrimer , differential pulse voltammetry , detection limit , aptamer , cyclic voltammetry , dielectric spectroscopy , electrochemistry , chemistry , electrode , oligonucleotide , combinatorial chemistry , materials science , nuclear chemistry , analytical chemistry (journal) , chromatography , polymer chemistry , dna , biochemistry , biology , genetics
Electrochemical aptasensing of APC was carried out using PAMAM dendrimer modified pencil graphite electrodes (PGEs) for the first time herein. Poly(amidoamine) dendrimer having 16 succinamic acid surface groups (generation 2, G2‐PS) modified PGEs were developed, and then were utilized for APC monitoring using differential pulse voltammetry, electrochemical impedance spectroscopy and cyclic voltammetry. The selectivity of single‐use aptasensor was tested against to other proteins; BSA and THR as well as to the affinity of APC binding to different DNA aptamer, or oligonucleotide. Voltammetric APC detection was also explored in a diluted fetal bovine serum resulting with a detection limit DL as 1.5 µg/mL.